![]() This email address is being protected from spambots. Dynamic Light Scattering (DLS) is an established and precise measurement technique for characterizing particle sizes in suspensions and emulsions. Molecular Weight of polymers dispersed in solution: On a sample volume of 10-40 µL, the instrument can determine a range between 1kDa to 25 MDa. It can analyse a wide range of suspension concentrations (0.1 ppm to 40% v/v depending on the sample type). It claims an accuracy of 0.12 µm.cm/V.s and a sensitivity of 10 mg/mL (BSA). Minimum sample volume required is 175 µL. Zeta Potential: It can measure wide range of zeta potentials for particles in the size range 1 nm – 100 µm, depending on the type of sample. It claims an accuracy of ☒% with a precision of ☑%. Particle Size and Molecular Size: It can measure size between 0.3 nm to 6 µm with a minimum volume 10 µL of sample suspension. A method of diagnosing a pathological condition by detecting microparticles in a sample of bodily fluid using dynamic light scattering (DLS) is disclosed. ![]() It can also be used to probe the behaviour of complex fluids such as concentrated polymer solutions. Dynamic Light Scattering (DLS) principle can be used to determine the size distribution profile of small particles in suspension or polymers in solution. When the potential is low, attraction exceeds repulsion and the dispersion will break and flocculate. For molecules and particles that are small enough, a high zeta potential will confer stability, i.e., the solution or dispersion will resist aggregation. The zeta potential indicates the degree of repulsion between adjacent, similarly charged particles (e.g., the vitamins) in a dispersion. The diffusion coefficient, and hence the hydrodynamic radii calculated from it, depends on the size and shape of macromolecules. The significance of zeta potential is that its value can be related to the stability of colloidal dispersions (e.g., a multivitamin syrup). Dynamic light scattering (DLS), also known as photon correlation spectroscopy (PCS), is a very powerful tool for studying the diffusion behaviour of macromolecules in solution. Dynamic light scattering (DLS), also known as photon correlation spectroscopy (PCS), is a very powerful tool for studying the diffusion behaviour of macromolecules in solution. It can determine particle size distribution, particle zeta potential (related to the magnitude of the electrical charge at the particle surface) and molecular weight of large polymeric substances dispersed in water. The DLS Particle Size and Zeta Potential Analyzer is a user-friendly system for colloidal, nanoparticulate and macromoleculer characterization.
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